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Th112 - Correlation of Urine Immune Cells with Renal Histological Features in Lupus Nephritis: Lessons from the Accelerating Medicines Partnership (AMP) in SLE Consortium

Thursday, June 22, 2023

7:30 AM – 7:30 PM

Joseph Mears – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Thomas Eisenhaure – Broad Institute of MIT and Harvard, Cambridge, MA, USA; Qian Xiao – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Paul Hoover – Broad Institute of MIT and Harvard, Cambridge, MA, USA; Deepak Rao – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Celine Berthier – University of Michigan, Ann Arbor, MI, USA; Andrea Fava – Johns Hopkins University, Baltimore, MD, USA; Siddarth Gurajala – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Saori Sakaue – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; William Apruzzese – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Jennifer Barnas – University of Rochester Medical Center, Rochester, NY, USA; Derek Fine – Johns Hopkins University, Baltimore, MD, USA; James Lederer – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Richard Furie – The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA; Anne Davidson – The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA; David Hildeman – University of Cincinnati College of Medicine, Cincinnati, OH, USA; Steve Woodle – University of Cincinnati College of Medicine, Cincinnati, OH, USA; Judith James – Oklahoma Medical Research Foundation, Oklahoma City, OK, USA; Joel Guthridge – Oklahoma Medical Research Foundation, Oklahoma City, OK, USA; Maria Dall'Era – University of California San Francisco, San Francisco, CA, USA; David Wofsy – University of California San Francisco, San Francisco, CA, USA; Peter Izmirly – New York University School of Medicine, New York, NY, USA; Michael Belmont – New York University School of Medicine, New York, NY, USA; Robert Clancy – New York University School of Medicine, New York, NY, USA; Diane Kamen – Medical University of South Carolina, Charleston, SC, USA; Chaim Putterman – Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, USA; Thomas Tuschl – Rockefeller University, New York, NY, USA; Maureen McMahon – University of California Los Angeles, Los Angeles, CA, USA; Jennifer Grossman – University of California Los Angeles, Los Angeles, CA, USA; Kenneth Kalunian – University of California San Diego School of Medicine, La Jolla, CA, USA; Fernanda Payan-Schober – Texas Tech University Health Sciences Center, El Paso, TX, USA; Mariko Ishimori – Cedars-Sinai Medical Center, Los Angeles, CA, USA; Michael Weisman – Cedars-Sinai Medical Center, Los Angeles, CA, USA; Matthias Kretzler – University of Michigan, Ann Arbor, MI, USA; Jeffrey Hodgin – University of Michigan, Ann Arbor, MI, USA; Michael Brenner – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Jennifer Anolik – University of Rochester Medical Center, Rochester, NY, USA; Michelle Petri – Johns Hopkins University, Baltimore, MD, USA; Jill Buyon – New York University School of Medicine, New York, NY, USA; Soumya Raychaudhuri – Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Nir Hacohen – Broad Institute of MIT and Harvard, Cambridge, MA, USA; Betty Diamond – The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA; Arnon Arazi – The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA

Rayan Najjar, MD, MPH

Clinician Researcher
University of Washington
seattle, Washington, United States
JM

Joseph Mears

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
TE

Thomas Eisenhaure

Broad Institute of MIT and Harvard, Cambridge, MA, USA
QX

Qian Xiao

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
PH

Paul Hoover

Broad Institute of MIT and Harvard, Cambridge, MA, USA
DR

Deepak Rao

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
CB

Celine Berthier

University of Michigan, Ann Arbor, MI, USA
AF

Andrea Fava

Johns Hopkins University, Baltimore, MD, USA
SG

Siddarth Gurajala

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
SS

Saori Sakaue

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
WA

William Apruzzese

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
JB

Jennifer Barnas

University of Rochester Medical Center, Rochester, NY, USA
DF

Derek Fine

Johns Hopkins University, Baltimore, MD, USA
JL

James Lederer

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
RF

Richard Furie

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA
AD

Anne Davidson

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA
DH

David Hildeman

University of Cincinnati College of Medicine, Cincinnati, OH, USA
SW

Steve Woodle

University of Cincinnati College of Medicine, Cincinnati, OH, USA
JJ

Judith James

Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
JG

Joel Guthridge

Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
MD

Maria Dall'Era

University of California San Francisco, San Francisco, CA, USA
DW

David Wofsy

University of California San Francisco, San Francisco, CA, USA
PI

Peter Izmirly

New York University School of Medicine, New York, NY, USA
MB

Michael Belmont

New York University School of Medicine, New York, NY, USA
RC

Robert Clancy

New York University School of Medicine, New York, NY, USA
DK

Diane Kamen

Medical University of South Carolina, Charleston, SC, USA
CP

Chaim Putterman

Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, USA
TT

Thomas Tuschl

Rockefeller University, New York, NY, USA
MM

Maureen McMahon

University of California Los Angeles, Los Angeles, CA, USA
JG

Jennifer Grossman

University of California Los Angeles, Los Angeles, CA, USA
KK

Kenneth Kalunian

University of California San Diego School of Medicine, La Jolla, CA, USA
FP

Fernanda Payan-Schober

Texas Tech University Health Sciences Center, El Paso, TX, USA
MI

Mariko Ishimori

Cedars-Sinai Medical Center, Los Angeles, CA, USA
MW

Michael Weisman

Cedars-Sinai Medical Center, Los Angeles, CA, USA
MK

Matthias Kretzler

University of Michigan, Ann Arbor, MI, USA
JH

Jeffrey Hodgin

University of Michigan, Ann Arbor, MI, USA
MB

Michael Brenner

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
JA

Jennifer Anolik

University of Rochester Medical Center, Rochester, NY, USA
MP

Michelle Petri

Johns Hopkins University, Baltimore, MD, USA
JB

Jill Buyon

New York University School of Medicine, New York, NY, USA
SR

Soumya Raychaudhuri

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
NH

Nir Hacohen

Broad Institute of MIT and Harvard, Cambridge, MA, USA
BD

Betty Diamond

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA
AA

Arnon Arazi

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA
JM

Joseph Mears

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
TE

Thomas Eisenhaure

Broad Institute of MIT and Harvard, Cambridge, MA, USA
QX

Qian Xiao

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
PH

Paul Hoover

Broad Institute of MIT and Harvard, Cambridge, MA, USA
DR

Deepak Rao

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
CB

Celine Berthier

University of Michigan, Ann Arbor, MI, USA
AF

Andrea Fava

Johns Hopkins University, Baltimore, MD, USA
SG

Siddarth Gurajala

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
SS

Saori Sakaue

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
WA

William Apruzzese

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
JB

Jennifer Barnas

University of Rochester Medical Center, Rochester, NY, USA
DF

Derek Fine

Johns Hopkins University, Baltimore, MD, USA
JL

James Lederer

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
RF

Richard Furie

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA
AD

Anne Davidson

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA
DH

David Hildeman

University of Cincinnati College of Medicine, Cincinnati, OH, USA
SW

Steve Woodle

University of Cincinnati College of Medicine, Cincinnati, OH, USA
JJ

Judith James

Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
JG

Joel Guthridge

Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
MD

Maria Dall'Era

University of California San Francisco, San Francisco, CA, USA
DW

David Wofsy

University of California San Francisco, San Francisco, CA, USA
PI

Peter Izmirly

New York University School of Medicine, New York, NY, USA
MB

Michael Belmont

New York University School of Medicine, New York, NY, USA
RC

Robert Clancy

New York University School of Medicine, New York, NY, USA
DK

Diane Kamen

Medical University of South Carolina, Charleston, SC, USA
CP

Chaim Putterman

Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, USA
TT

Thomas Tuschl

Rockefeller University, New York, NY, USA
MM

Maureen McMahon

University of California Los Angeles, Los Angeles, CA, USA
JG

Jennifer Grossman

University of California Los Angeles, Los Angeles, CA, USA
KK

Kenneth Kalunian

University of California San Diego School of Medicine, La Jolla, CA, USA
FP

Fernanda Payan-Schober

Texas Tech University Health Sciences Center, El Paso, TX, USA
MI

Mariko Ishimori

Cedars-Sinai Medical Center, Los Angeles, CA, USA
MW

Michael Weisman

Cedars-Sinai Medical Center, Los Angeles, CA, USA
MK

Matthias Kretzler

University of Michigan, Ann Arbor, MI, USA
JH

Jeffrey Hodgin

University of Michigan, Ann Arbor, MI, USA
MB

Michael Brenner

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
JA

Jennifer Anolik

University of Rochester Medical Center, Rochester, NY, USA
MP

Michelle Petri

Johns Hopkins University, Baltimore, MD, USA
JB

Jill Buyon

New York University School of Medicine, New York, NY, USA
SR

Soumya Raychaudhuri

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA
NH

Nir Hacohen

Broad Institute of MIT and Harvard, Cambridge, MA, USA
BD

Betty Diamond

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA
AA

Arnon Arazi

The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA

Abstract Text: Lupus nephritis (LN) is a complex autoimmune disease with unknown cause that is associated with severe morbidity and mortality. Recent research suggested a benefit of serial kidney biopsies in managing LN. Studying immune cells in the urine of LN patients offers the prospect of minimizing the need for invasive kidney biopsies. We performed analysis of single cell RNA-sequencing data of urine immune cells collected from patients with active LN. We identified 101,787 high quality immune cells from 146 patients including myeloid, dendritic, B, T, NK, and dividing cells. The median number of cells per patient was 315 (IQR 42-1,071). The majority of cells were myeloid (91,215 cells, 89.6%); Still, most immune cell subsets identified in the kidneys of LN patients, including lymphocyte subsets, were also found in the urine. Approximately 39% of urine samples yielded higher numbers of immune cells compared to the corresponding kidney biopsies. Proliferative and mixed nephritis were associated with higher percentages of phagocytic myeloid cells (Kruskal Wallis q value < 0.001). The NIH Activity Index positively correlated with phagocytic myeloid fractions (Spearman rho=0.40, q value=0.002) and negatively correlated with that of dendritic cells (Spearman rho=-0.29, q value=0.030); while the NIH Chronicity Index correlated positively with B cells (Spearman rho=0.32, q value=0.012), negatively with phagocytic myeloid cells (Spearman rho=-0.28, q value=0.035), and positively with inflammatory myeloid cells (Spearman rho=0.32, q value=0.012). This work describes the complex immune cells present in urine during LN, and paves the way for additional work to predict renal histological features from urine samples.