Tu168 - Impact of slc11a1 Gene Variants on Host Response and Tissue Repair After Ti Implantation

michelle azevedo – Bauru School of Dentistry-University of São Paulo; priscila colavite – Bauru School of Dentistry-University of São Paulo; angelica fonseca – Bauru School of Dentistry-University of São Paulo; carolina Mortari – Bauru School of Dentistry-University of São Paulo; andré tabanez – Bauru School of Dentistry-University of São Paulo; andrea borrego – Instituto Butantan; orlando ribeiro filho – Instituto Butantan; ana paula trombone – UNISAGRADO; gustavo garlet – Bauru School of Dentistry-University of São Paulo

Abstract Text: The role of host inflammatory immune response to biomaterials grafting remains unclear. Slc11a1 is a modulator inflammatory response intensity, being one of the factors responsible for the hyper/hyper-responsiveness of AIRmax/AIRmin mice strains. To address the role of host response to biomaterial implantation, Ti devices were implanted in the subcutaneous tissue of AIRmin and AIRmax mice, followed by histomorphometric and immunological analysis. When comparing the AIRmin vs AIRmax groups, a higher density of blood clot density was observed in AIRmax group at 1 and 3 days. AIRmin animals showed higher fibroblasts and collagen fibers density in 1 to 7 days period, while AIRmax strain present a higher density at 14 days. The connective tissue showed statistical difference in the periods of 7 and 14 days where AIRmin has higher density in 7 days and AIRmax in 14 days. Regarding inflammatory cells, AIRmax strain presented a higher density at 1,7 and 14 days, as well as for giant cells in the period of 3d. The immunohistochemical analysis revealed increased GR1 counts at 1 and 3 days in AIRmax strain, while CD3 and CD206 were upregulated in this group at 14d time point. Our results demonstrate AIRmax strain mice presents a stronger pro-inflammatory responsiveness to Ti grafting when compared with AIRmin strain. The exacerbated host response is associated with delayed tissue repair at the grafting area, while a moderate responsiveness results in a more favorable repair outcome, suggesting that translational strategies to tune host response may be applied to improve biomaterials grafting outcome.