Abstract Text: The acute phase response is a complex reaction triggered by the onset of various critical conditions such as trauma or tissue damage, inflammation, stress, and neoplasia. Such response is characterized by an increase of liver-secreted proteins, known as acute phase proteins. One of them is fibrinogen, an important glycoprotein involved in coagulation. Fibrinolysis is a naturally occurring process that controls blood coagulation and the breakdown of clots to restore blood flow. BioLegend has developed two Multiplex Panels using flow cytometry for quantifying critical acute phase and fibrinolysis proteins. LEGENDplex™ Human Acute Phase Panel 1 is designed for the simultaneous quantification of 8 key acute phase molecules including α2-microglobulin, α1-Acid Glycoprotein, Haptoglobin, α1-antitrypsin, Ceruloplasmin, Fibrinogen, Prothrombin, and Serum Amyloid P Component in blood and cell culture supernatant samples. LEGENDplex™ Human Fibrinolysis Panel allows the measurement of 5 human proteins: Fibrinogen, Antithrombin, Plasminogen, Prothrombin, and Factor XIII in plasma samples. The antibody pairs used in these assays are highly specific and validated on biosamples for their ability to detect native proteins. The Panels are thoroughly validated for dilution linearity, cross-reactivity, and inter- and intra-assay precision, and offer greater efficiency and broader dynamic ranges than the conventional ELISA method. These panels are valuable research tools for studying the pathology of related diseases, development and testing of therapeutics, monitoring of the condition, and risk prediction in cardiovascular diseases and many other disorders.
